LET5821 - Biotecnologia Aplicada à Entomologia

Carga Horária

Docentes responsáveis
Fernando Luis Consoli

Objetivo
A disciplina tem por objetivo abordar técnicas e avanços tecnológicos no estudo e exploração biotecnológica de insetos e suas associações, envolvendo assim diversas áreas de conhecimento relevantes ao desenvolvimento da Entomologia Agrícola, voltadas principalmente ao uso de estratégias contemporâneas que permitiriam a utilização de estratégias sustentáveis de manejo de pragas. Visa, assim, proporcionar uma visão multidisciplinar de ensino e pesquisa e permitir ao aluno de Doutorado interação inter e multi disciplinar.

Conteúdo
Uma breve revisão da estrutura e funcionamento celular; Abordagens para amplificação do DNA e sequenciamento de DNA/RNA; Isolamento de moléculas - eletroforese; Isolamento de moléculas - cromatografia; Abordagens químicas e moleculares para o estudo da diversidade de insetos; Abordagens moleculares no estudo de genética populacional de insetos; Abordagens no estudo da expressão gênica diferencial; Explorando o genoma de insetos; Explorando o transcritoma de insetos; Explorando o metaboloma de insetos; Explorando o proteoma de insetos; Explorando as interações em insetos; Controle de insetos - RNA de interferência; Controle de insetos - transgenia; Controle de insetos - paratransgenia; Controle de insetos - edição gênica, CRISPR; Princípios e aplicações da nanobiotecnologia.

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